![]() ![]() Provided care is taken in cases where the deletion is limited to a single exon, the multiplex PCR appears to be an efficient and useful alternative to conventional Southern blot analysis for detecting deletions during the prenatal and postnatal diagnosis of DMD. The Southern blotting technique was named after. Southern. It is a multi-step process, that begins with the electrophoresis of DNA, transfer of DNA fragments into nitrocellulose strip, and exposing those fragments to a DNA probe labeled with a radioactive or chemical tag. One of the most useful techniques for analyzing a gene at the level of genomic DNA is Southern blotting, named for its originator, E.M. Thirteen (93%) of the 14 DMD families with deletions detected by Southern blotting were also confirmed by the multiplex PCR. Southern blotting is a molecular technique to find target DNA sequences in a sample. Southern blot analysis detected deletions in 14 (64%) out of 22 families. Deletions were detected in 14 (52%) out of 27 DMD families by the PCR. Useful to detect defective proteins (Prions disease). and another 10 regions by the method of Beggs et al. The genomic Southern Blot procedure involves four major steps: restriction digestion of genomic DNA (gDNA) and electrophoresis, transfer to membrane, hybridization, and detection. Western blotting technique is the confirmatory test for HIV. Southern blot hybridization of genomic DNA from 66 tail biopsies using a. Susceptibility testing by polymerase chain reaction DNA quan- titation: A method to measure drug resis- tance of human immunodeficiency virus type 1 isolates. A total of 19 regions were amplified by the PCR to detect deletions, 9 regions by the method of Chamberlain et al. Division of Applied Plianiiacology Research, Ofice of Testing and Research. We used five dystrophin cDNA probes for deletion analysis. Thirty males with DMD from 27 Japanese families were studied by the multiplex PCR, and 24 of them were also investigated by Southern blot analysis. We compared the efficacy of the multiplex PCR with that of the cDNA analysis for detection of deletions of the DMD gene in the Japanese patients. ![]()
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